Part:BBa_K143032:Design

EpsE Molecular Clutch Gene of B. subtilis

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 762
  Illegal AgeI site found at 512
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The epsE(aka yveO) sequence was located in the B. subtilis chromosome#1 and the PstI restriction site removed before synthesis by GeneArt

Source

The epsE gene sequence was taken from the B. subtilis chromosome and was synthesised by GeneArt.

References

<biblio>

1. 1 pmid=9384377

</biblio>